Part:BBa_K5333000:Experience

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Applications of BBa_K5333000

The original sequence build for the gBlock is encoded here

pET28-His-GR TCCTTTCGGGCTTTGTTACTTCTGATGAAACAGAAGTTTTTTGATATTGCCATTTGAATATTTTGGTATCTGATTGGTGATGATTTCAGCTAACATCTCGGGGAATTCAATACTCATGGTCTTATCCAAGAATGTCTGGAAGCAATAGTTAAGCAGATTTTCGACCACTTCATGCATAGAATCCAAGAGTTTTGTCAGTTGGTAAAAGCGCTGCCAGTTCTGGCTGGAGTTGCCTTCCCGTTTGACAATGGCTTTGCCCAGCTCTTTGATGTAGGTCATGCGAATTTCATCAAACAGCTCCTGGCTCTTCAGACCGTCCTTCGGGACTGAAGAGAGAAGCAGTAAGGTTTTCATACAGAGATATTCTTCATACGATACCTGAAGACGATGCAACTCCGAGGAAACATACAGCATGTGTTTACATTGGTCGTACATGCACGGCAGGGTCATGCGCTGCTCATTAATAATCAGATCAGGAGCAAAGCACAGCAGGTTTGCACTTGACTGACGATAGCTGCGCCACCCCAGCGCAAAGGCCATAAGAAACATCCAGGAGTACTGCAGCAAGGTCATTTGGTCATCCAGGTGTAAGTTGCGGAAACCCGGGATCGCTTTTGCCCATTTCACCGCTGCAATCACTTGACGCCCTCCCAGCATGTTCAGCGTAGTCATGATCCGCCAAGTAGAGTCCGGAACCGAGCTATCGTAACCGGCATATAACACTTCAGGTTCAATAACTTCCAACAGACTCACCAGCGTCGGCGTGAGTTGTGGTAACGTCGCGGGAACGCTGCCACGCGGTACCAGGCCATGATGGTGGTGATGGTGACCTTTTTTCATGGTATATCTCCTTCT

The original sequence is functional, however the modified sequence in the parts registry is the subsitute that fits the guidelines, as the original sequence includes illegal restriction enzyme cleavage sites.

Even thought the sequence used is directly derived from the literature (He et al.,2014) includes specific mutations to improve the solubility of the protein, we still encounter challenges to obtain the protein in the soluble fraction according to our laboratory results.

User Reviews

UNIQ6c82f4254415a474-partinfo-00000000-QINU UNIQ6c82f4254415a474-partinfo-00000001-QINU