Composite

Part:BBa_K5328012

Designed by: zonghuan li   Group: iGEM24_BJWZ-3-China   (2024-09-30)


PGlu+MazF

Combining the glucose operon with the cell endotoxin gene allows for sensing changes in glucose concentration in the external environment, achieving the effect of suicide to prevent dispersion. It has been optimized it make it suitable for expression in Lactobacillus rhamnosus. And it prevents the strain from rupturing and reduces environmental pollution after killing it.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 26
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 26
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 26
    Illegal NgoMIV site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None