Composite
Part:BBa_K5328012
Designed by: zonghuan li Group: iGEM24_BJWZ-3-China (2024-09-30)
PGlu+MazF
Combining the glucose operon with the cell endotoxin gene allows for sensing changes in glucose concentration in the external environment, achieving the effect of suicide to prevent dispersion. It has been optimized it make it suitable for expression in Lactobacillus rhamnosus. And it prevents the strain from rupturing and reduces environmental pollution after killing it.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 26
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 26
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 26
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 26
Illegal NgoMIV site found at 715 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |