Composite
Part:BBa_K5328009
Designed by: zonghuan li Group: iGEM24_BJWZ-3-China (2024-09-30)
paT7P-1
Separate the T7RNA polymerase from positions 564-565 and connect a segment of the vvd domain to form two fusion proteins that coexist in the cell. When receiving blue light, VVD forms a homodimer, causing the two parts of T7RNA polymerase to bind and restore their biological activity
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 18
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 524
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2260
[edit]
Categories
Parameters
None |