Composite

Part:BBa_K5328009

Designed by: zonghuan li   Group: iGEM24_BJWZ-3-China   (2024-09-30)


paT7P-1

Separate the T7RNA polymerase from positions 564-565 and connect a segment of the vvd domain to form two fusion proteins that coexist in the cell. When receiving blue light, VVD forms a homodimer, causing the two parts of T7RNA polymerase to bind and restore their biological activity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2260


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Categories
Parameters
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