Coding

Part:BBa_K5328001

Designed by: zonghuan li   Group: iGEM24_BJWZ-3-China   (2024-09-30)


The basic structure of this component is laccase from B. subtilis, which can degrade aflatoxin.

The basic structure of this component is laccase from B. subtilis, which can degrade aflatoxin. It has been mutated three related sites based on relevant literature, significantly improving its degradation efficiency and stability.We have optimized it to be suitable for expression in Lactobacillus rhamnosus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1477
    Illegal AgeI site found at 117
    Illegal AgeI site found at 1414
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None