Part:BBa_K5327024
NAD(P) transhydrogenase subunit beta
Function:
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Usage and Biology
Genome localization:NC_000913.3
Expression diagram:
- Fig 1. The expression diagram of NAD(P) transhydrogenase subunit beta
Corresponding enzyme structure:
- Fig 2. The corresponding enzyme structure of NAD(P) transhydrogenase subunit beta
The PCR result :
- Fig 3. The PCR result of NAD(P) transhydrogenase subunit beta
Subcellular localization:
Located in the cell inner membrane
- Fig 4. The subcellular localization of NAD(P) transhydrogenase subunit beta
Design Notes
The design of the NAD(P) transhydrogenase subunit beta gene is based on the coding sequence from Saccharomyces cerevisiae and has undergone codon optimization to enhance its expression in yeast. This enzyme plays a critical role in the transhydrogenation process between NADH and NADP, coupling this reaction with respiration and ATP hydrolysis, and functions as a proton pump across the membrane. The gene is expressed using the ADH1 promoter (ADH1p) and the TDH2 terminator (TDH2t) to ensure robust expression levels and mRNA stability in yeast. The optimized gene is incorporated into an expression vector and introduced into the Saccharomyces cerevisiae S288C strain via homologous recombination, with screening and expression validation conducted using a defective strain. This design aims to enhance the functionality of NAD(P) transhydrogenase in yeast, thereby improving cellular energy metabolism and providing a more efficient platform for metabolic engineering applications.
Plasmid
- Fig 1. The plasmid expression of NAD(P) transhydrogenase subunit beta
Source
Arabidopsis thaliana
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 82
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