Coding

Part:BBa_K5325000:Design

Designed by: Hien Le, Dang Minh Anh   Group: iGEM24_MichiganState   (2024-10-01)


ABO_plaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of a C-terminal FLAG tag that aids protein purification and visualization on Western Blot.
Original DNA sequence was codon optimized for expression in E. coli and Shewanella oneidensis MR-1.

Source

The part originated from Alcanivorax borkumensis SK2 sequence, which is an identical sequence to the PLA depolymerase DNA sequence mentioned in the referenced study.
NCBI ID and link to NCBI profile: NC_008260.1

References

Hajighasemi, M., Nocek, B.P., et al. (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039