Composite
Part:BBa_K5324015
Designed by: Sebastian Nolte Group: iGEM24_Alma (2024-09-26)
Destructin-1 Expression Device
This composite part produces Destructin-1, a collagen degrading serine protease normally produced by P. destructans. The destructin-1 sequence on its C-terminal has a histidine purification tag connected with a linker. The composite is reinforced with an Anderson promoter, RBS, and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 102 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 879
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
protein | Destructin-1 |
protein_out | Destructin-1 |