Composite

Part:BBa_K5324011

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-26)


Aprotinin Secretion Device

This composite part produces and secretes aprotinin, a serine protease inhibitor. The aprotinin sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
proteinAprotinin
protein_outAprotinin