Composite

Part:BBa_K5323990:Design

Designed by: Yidan Hu   Group: iGEM24_CUG-China   (2024-10-01)


Ptac-RBS-AFE_0053-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2256
    Illegal AgeI site found at 2419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1680


Design Notes

Regulate the expression of AFE_0053(BBa_5323000).



Source

AAssembled by BBa_K180000 , BBa_J34801,BBa_K5323000 and BBa_B0015.

References

Ruiz, L. M., Castro, M., Barriga, A., Jerez, C. A., & Guiliani, N. (2012). The extremophile Acidithiobacillus ferrooxidans possesses ac‐di‐GMP signalling pathway that could play a significant role during bioleaching of minerals. Letters in applied microbiology, 54(2), 133-139.