Composite

Part:BBa_K5323896:Design

Designed by: Yidan Hu   Group: iGEM24_CUG-China   (2024-10-01)


RBS-AFE_1373


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 680
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Enhancing the expression of AFE_1373 by using strong RBS.


Source

This part is from Acidithiobacillus ferrooxidans ATCC 23270, assembled by BBa_J34801 and BBa_K5323999.

References

Wei, W., Zhu, T., Wang, Y., Yang, H., Hao, Z., Chen, P. R., & Zhao, J. (2012). Engineering a gold-specific regulon for cell-based visual detection and recovery of gold. Chemical Science, 3(6), 1780-1784.