Part:BBa_K5323893:Design

P_tac-RBS-golS-P_gol-RBS-cI-TT-PR-RBS-mazF-TT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1635
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3721
  Illegal AgeI site found at 1969
  Illegal AgeI site found at 2318
  Illegal AgeI site found at 3256
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

A component that determines whether mazF gene is expressed or not by a gold detector.

Source

A composite part assembled by RBS(BBa_J34801), golS (BBa_K4468005), P_gol(BBa_K4468001), cI (BBa_R1051), P_tac(BBa_K180000), TT(BBa_B0015), mazF(BBa_K1096002) and P_R(BBa_K2229450).