Part:BBa_K5322037

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7

Usage and Biology

In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments.

Figure 1-1 Plasmid pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 683
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 552
  Illegal NheI site found at 702
  Illegal NheI site found at 725
  Illegal NheI site found at 1475
  Illegal SpeI site found at 683
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 683
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 683
  
• 1000
  COMPATIBLE WITH RFC[1000]