Coding

Part:BBa_K5322036

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)


Cleavable Flexible Protein Linker（GISS+TEV site）

Usage and Biology

In order to prove the success of the surface membrane display system, we designed a cleavable flexible protein linker: GISS+TEV site, which can be treated with TEV enzyme to separate the passenger protein displayed outside the membrane from OmpA, which is used to verify the success of the surface membrane display.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None