Coding

Part:BBa_K5322033:Design

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-09-30)


Programmed Cell Death 1 Ligand 1 [Mus musculus]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 253
    Illegal PstI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 295
    Illegal NgoMIV site found at 684
    Illegal AgeI site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After obtaining the mRNA sequence, we converted it into double-stranded DNA. Subsequently, we performed codon optimization based on the codon usage table for Escherichia coli.


Source

Escherichia coli chromosome 19

References