Coding

Part:BBa_K5321014:Design

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)


SA_nPPVp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 853
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 853
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 853
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 853
    Illegal NgoMIV site found at 508
    Illegal AgeI site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fragments can be obtained by gene synthesis.


Source

Streptavidin sequence came from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/X03591.1?report=genbank), while we designed the fused protein.

References