Coding

Part:BBa_K5321007:Design

Designed by: Xiangkai Jin   Group: iGEM24_Peking   (2024-09-21)


Plum pox virus protease (PPVp), codon optimized for E. coli, 6x His tagged


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 712
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene synthesis should be performed to obtain this coding sequence.


Source

Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli.

References