Generator

Part:BBa_K5319688

Designed by: Zhang Liting   Group: iGEM24_ZJU-China   (2024-10-01)


VioE expression circuit

The device consists of a salicylic acid specific promoter Psal(BBa_K5319686) and a downstream VioE(BBa_K771203). Salicylic acid binds to its specific receptor nahR-Q168R (BBa_K5319685), inducing Psal to express downstream VioE, thus staining the cloth green. With salicylic acid, we can precisely control the coloring effect of the pigment on the fabric.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 301
    Illegal AgeI site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 531
    Illegal BsaI site found at 975


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Categories
Parameters
None