RNA

Part:BBa_K5319680

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-10-01)


47CAG-2boxB

It's been proved that CAG triple-ribonucleotide (rCAG) repeats will lead to intracellular spatial separation and form a coheseive bead. Besides, box B sequences and λN peptide specifically combine with each other. With these two elements, researchers have build RNA scaffold and prove that it can achieve the effect of enzyme co-localization.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 260
    Illegal NheI site found at 306
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221
    Illegal BsaI.rc site found at 386


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Categories
Parameters
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