Plasmid

Part:BBa_K5319672

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


lacUV5-J2/3-RS

It's a modified ribozyme of HYER, hydrolytic endonucleolytic ribozymes, which are identified from bacterial group II-C introns and hydrolyse DNA at specific sites, with a point mutation G338A attempting to destroy the DNA hydrolysis activity and an extra 14-nt recognition site(Recruiting Sequence) while retaining a target recognition site of 6nt(AAGACA). The promoter lacUV5 is added upstream.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1209
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1209
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1209
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 425
    Illegal XbaI site found at 843
    Illegal PstI site found at 1209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2546
    Illegal SapI site found at 1463


[edit]
Categories
Parameters
None