RNA

Part:BBa_K5319669

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


HYER-J2/3

It's a modified ribozyme of HYER, hydrolytic endonucleolytic ribozymes, which are identified from bacterial group II-C introns and hydrolyse DNA at specific sites, with a point mutation attempting to destroy the DNA hydrolysis activity while retaining a recognition site of 6nt(AAGACA). The point mutation was G338A.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 361
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None