Coding

Part:BBa_K5317013:Design

Designed by: Lisa Marie de Sousa Miranda   Group: iGEM24_Hannover   (2024-09-15)


PknB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1213
    Illegal SpeI site found at 1876
    Illegal PstI site found at 638
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1876
    Illegal PstI site found at 638
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 888
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1213
    Illegal SpeI site found at 1876
    Illegal PstI site found at 638
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1213
    Illegal SpeI site found at 1876
    Illegal PstI site found at 638
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon-optimized for the use in human cell lines. Avoidance of frame shifts. Important to considre start and stop codon.


Source

The gene sequence from PknB was aquired from S. aureusand is codon-optimized for the use in human cell lines. The sequence was synthesized for further use.

References