Reporter

Part:BBa_K5316020

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


H2B-mCherry

Targeting reporters to specific cellular locations can be very useful for a wide range of applications. In this case, the reporter mCherry is targeted to the nucleus by the creation of a fusion protein between the H2B histone and mCherry.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None