Regulatory

Part:BBa_K5316019

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


CMV Promoter

The human cytomegalovirus immediate early (CMV) promoter is a strong, constitutive, and ubiquitous promoter commonly used in research to drive gene expression in a wide range of mammalian cell types. Renowned for its potency, the CMV promoter provides robust expression levels, although its strength may vary between different cell types. This makes it suitable for many applications, including transient and stable transfection experiments.

The CMV promoter is highly effective for achieving high-level gene expression, making it ideal for experiments that require significant protein production, such as in therapeutic vector design, CRISPR-Cas9 systems, and recombinant protein generation. It operates independently of chromosomal position, ensuring consistent gene expression, though some variability in expression strength may be observed depending on the specific cellular environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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