Signalling

Part:BBa_K5316015

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


Lck signal peptide

The Lck signal peptide is a membrane-targeting sequence derived from the lymphocyte-specific protein tyrosine kinase (Lck). When fused to a protein of interest, this short peptide directs the protein to the plasma membrane by facilitating its attachment to the inner leaflet via lipid modifications, such as myristoylation or palmitoylation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 86
    Illegal BamHI site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
    Illegal AgeI site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None