DNA

Part:BBa_K5316010

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


ORF_Stuffer

The ORF Stuffer, composed of amino acids 2-83 of E. coli β-galactosidase, functions as a negative open reading frame (ORF) control in molecular biology experiments.

This stuffer sequence is specifically designed to occupy vector space without generating a functional protein, enabling researchers to evaluate the effects of true gene expression without interference from background activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None