Coding

Part:BBa_K5316006:Design

Designed by: Santiago Ramos Bartolome and Andrea Camí Bonet   Group: iGEM24_Barcelona-UB   (2024-09-30)


hBDNF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 710
    Illegal PstI site found at 831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 710
    Illegal PstI site found at 831
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 710
    Illegal PstI site found at 831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 710
    Illegal PstI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iGEM Barcelona-UB team of 2024 adapted the original hBDNF sequence from Fulgenzi et al. to be Type IIS compatible.

BsaI.rc and SpaI.rc recognition sites, at positions 793 and 448 respectively, were removed by changing the codon to a synonym one, maintaining the amino acid and therefore its functionality.



Source

Sequence sourced from: Nat Commun. 2020 Apr 23;11(1):1950: https://pubmed.ncbi.nlm.nih.gov/32327658/

BDNF functions and interaction pathways sourced from: Bali Med J. [S.l.], v. 5, n. 2, may 2016: https://ojs.unud.ac.id/index.php/bmj/article/download/21797/14420


References