Part:BBa_K5316002
EF1A promoter
The human eukaryotic translation elongation factor 1 α1 (EF1A) promoter is a strong, constitutive, and ubiquitous mammalian promoter widely used in research for driving the expression of genes in a variety of mammalian cell types. It is known for its ability to provide stable and high-level gene expression, making it ideal for both transient and stable transfection experiments. EF1A is active in most cell types, including both dividing and non-dividing cells, which increases its versatility compared to other promoters that may be tissue- or cell-cycle specific.
This promoter is particularly useful for experiments where consistent and prolonged gene expression is desired, such as in the development of therapeutic vectors, CRISPR-Cas9 systems, and recombinant protein production. It functions by driving transcription of downstream genes under its control in a manner largely unaffected by the chromosomal position, ensuring robust gene expression.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 313
Illegal PstI site found at 818 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 313
Illegal PstI site found at 818 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 567
Illegal XhoI site found at 966 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 313
Illegal PstI site found at 818 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 313
Illegal PstI site found at 818
Illegal NgoMIV site found at 701
Illegal AgeI site found at 79 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 721
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