Part:BBa_K5313015:Design
frmA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 193
Illegal AgeI site found at 517 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
When designing the expression system for the frmA gene, we focused on regulating expression levels and managing the effects of toxic substances on the host cells. Since formaldehyde is highly toxic, promoter optimization for frmA was necessary in high-concentration formaldehyde degradation experiments to ensure the gene product effectively handles formaldehyde accumulation. Additionally, frmA protein expression could impose a metabolic burden on the host cells, so we selected a mild expression system to minimize stress. Codon optimization was also considered to enhance the gene's expression efficiency in E. coli.
Source
The frmA gene is derived from the genome of Escherichia coli. This gene is naturally present in wild-type E. coli and is responsible for the metabolism of formaldehyde. The gene sequence can be obtained through genomic sequencing or retrieved from public databases, such as NCBI, which provides detailed information on the frmA gene.