Composite

Part:BBa_K5311021:Design

Designed by: carlos Pérez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_026 + RNA thermometer ForU + CAP 10-3 Endolysin + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 705
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 453


Design Notes

For this composite part we have codon optimized the CDS for the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.


Source

This part was created by using the corresponding sequences on this registry: MG10:BBa_K115000; RNA Thermometer: Part:BBa_K115002; Endolysin: Part:BBa_K5311006 and the His-tag corresponds to a sequence of 6 Hystidines

References