Composite

Part:BBa_K5311021

Designed by: carlos Pérez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_026 + RNA thermometer ForU + CAP 10-3 Endolysin + His-tag

Genetic construct created to produce endolysin to induce cell death in C. acnes. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 705
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 453


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Categories
Parameters
None