Composite

Part:BBa_K5311019

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_026 + RNA thermometer ForU + mCherry + His-tag

Genetic construct created to produce mCherry to induce fluorescence in C. acnes. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 612
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 612
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None