Composite

Part:BBa_K5311014

Designed by: Carlos Perez de Arenaza and Jose Luis Perez   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_010 + RBS cell free MJ + CAP 10-3 Endolysin+ His-tag

Genetic construct created to produce Cry toxin 3Aa constitutively in C. acnes and E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
    Illegal NgoMIV site found at 523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 271


[edit]
Categories
Parameters
None