Composite

Part:BBa_K5311012

Designed by: carlos Pérez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_010 + RBS cell free MJ + Cry4Ba + RoxP + His-tag

Genetic construct created to produce Cry toxin 4Ba constitutively in C. acnes and E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1686
    Illegal BglII site found at 2916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 3407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1517
    Illegal BsaI.rc site found at 3620


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Categories
Parameters
None