Coding
sfGFP

Part:BBa_K5311007

Designed by: Carlos Perez de Arenaza and Jose Luis Perez   Group: iGEM24_UPF-Barcelona   (2024-09-24)


Super folded Green fluorescent protein

The sfGFP is a variant of the Green Fluorescent Protein (GFP) engineered for enhanced folding efficiency. It was developed to overcome issues related to protein misfolding that commonly affect GFP in some environments, such as during expression in complex protein fusions, sfGFP includes mutations that improve its folding kinetics, making it more stable and fluorescent even under denaturing conditions which allows it to be a reliable marker in experiments requiring protein tracking or visualization in vivo [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
chassisCutibacterium acnes and Escherichia Coli