Coding

Part:BBa_K5310050

Designed by: Elias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-30)


MOG-CAAR-v1

This composite part is made up of the following protein domains: EF1a promoter, Igk signal peptide, MOG (aa 35-55) autoantibody binding domain, short stable GS linker, CD8a hinge and transmembrane, CD28 signalling, 4-1-BB signalling ,CD3z signalling domain and a regulatory domain (polyA signal and stop codons). All these domains result in a chimeric protein that can recognise and bind to anti-MOG antibodies and produce cell responses. This makes the receptor ideal for halting disease progression in multiple sclerosis, if we employ it in a manner similar to traditional CAR-T cells

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1548
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 643
    Illegal BglII site found at 1610
    Illegal XhoI site found at 1042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1548
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1548
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 151
    Illegal AgeI site found at 1798
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None