Coding

Part:BBa_K531004:Design

Designed by: Nora Kostow   Group: iGEM11_Grinnell   (2011-07-11)


dspB from Actinobacillus actinomycetemcomitansis optimized for Caulobacter crescentus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 969
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 770


Design Notes

The WT gene has a low GC content ~40%. Caulobacter crescentus is GC rich and prefers a high GC content. Therefore, we optimized the GC content by comparing the codons in the wild type dspB gene to their corresponding amino acid codons that are most expressed in Caulobacter. We also eliminated the stop codon at the end of dspB because we wanted to add another gene to it that also needed to be transcribed.


Source

Kaplan, J., Ragunath, C., Ramasubbu, N., and Fine, D. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology. 2003 August; 185(16): 4693-4698.

References