Protein_Domain

Part:BBa_K5310011

Designed by: Ilias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-30)


CD3z signalling domain

CD247, known as CD3-zeta (encoded by BBa_K5310011) is the cytoplasmic domain used in all approved CAR T-cell therapies. Deriving from the transmembrane domain of TCRs, it contains Immunoreceptor Tyrosine-based Activation Motifs (ITAMs), a sequence necessary for signal transduction. When the ligand recognises the receptor, tyrosine residues in ITAMs are phosphorylated by a member of the Src tyrosine kinase family. ITAMs then attract ZAP-kinases which are also phosphorylated and activated, leading to downstream signaling. This results in Ras activation, Ca2+ mobilization, actin cytoskeleton reorganization and transcription factor activation which ultimately leads to T-cell activation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None