Coding
Part:BBa_K5306005:Design
Designed by: Iulia Beres Group: iGEM24_MSP-Maastricht (2024-09-26)
Design Considerations
This coding sequence for nasA has been obtained from the organism Klebsiella oxytoca m5al and has been codon optimized specifically for our chassis organism Vibrio natriegens using Benchlingand a personal codon optimization table that can be found on our wiki Codon Optimization Vibrio natriegensaccording to codon frequency taken from Lee et al. (2019).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 230
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1011
Illegal PstI site found at 230 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2423
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 230
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 230
Illegal AgeI site found at 1797 - 1000COMPATIBLE WITH RFC[1000]
References
- Lee, H. H., Ostrov, N., Wong, B. G., Gold, M. A., Khalil, A. S., & Church, G. M. (2019). Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Nature Microbiology, 4(7), 1105–1113. https://doi.org/10.1038/s41564-019-0423-8
- Benchling. (2023). Cloud-Based Informatics Platform for Life Sciences R&D. Benchling. https://www.benchling.com/