Plasmid

Part:BBa_K5303114

Designed by: Dongxun Li   Group: iGEM24_NNU-CHINA   (2024-09-27)


ptDonor

PtDonor carries the RChemA gene. And it includes RE-cargo-LE, allowing transposase to recognize and cleave RChemA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 421
    Illegal EcoRI site found at 3190
    Illegal EcoRI site found at 3962
    Illegal XbaI site found at 2451
    Illegal XbaI site found at 3788
    Illegal XbaI site found at 3847
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 421
    Illegal EcoRI site found at 3190
    Illegal EcoRI site found at 3962
    Illegal NheI site found at 5114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 421
    Illegal EcoRI site found at 3190
    Illegal EcoRI site found at 3962
    Illegal BamHI site found at 1375
    Illegal BamHI site found at 2023
    Illegal BamHI site found at 2457
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 421
    Illegal EcoRI site found at 3190
    Illegal EcoRI site found at 3962
    Illegal XbaI site found at 2451
    Illegal XbaI site found at 3788
    Illegal XbaI site found at 3847
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 421
    Illegal EcoRI site found at 3190
    Illegal EcoRI site found at 3962
    Illegal XbaI site found at 2451
    Illegal XbaI site found at 3788
    Illegal XbaI site found at 3847
    Illegal NgoMIV site found at 1349
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 4704
    Illegal AgeI site found at 5028
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None