Plasmid
Part:BBa_K5303114
Designed by: Dongxun Li Group: iGEM24_NNU-CHINA (2024-09-27)
ptDonor
PtDonor carries the RChemA gene. And it includes RE-cargo-LE, allowing transposase to recognize and cleave RChemA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 421
Illegal EcoRI site found at 3190
Illegal EcoRI site found at 3962
Illegal XbaI site found at 2451
Illegal XbaI site found at 3788
Illegal XbaI site found at 3847 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 421
Illegal EcoRI site found at 3190
Illegal EcoRI site found at 3962
Illegal NheI site found at 5114 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 421
Illegal EcoRI site found at 3190
Illegal EcoRI site found at 3962
Illegal BamHI site found at 1375
Illegal BamHI site found at 2023
Illegal BamHI site found at 2457 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 421
Illegal EcoRI site found at 3190
Illegal EcoRI site found at 3962
Illegal XbaI site found at 2451
Illegal XbaI site found at 3788
Illegal XbaI site found at 3847 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 421
Illegal EcoRI site found at 3190
Illegal EcoRI site found at 3962
Illegal XbaI site found at 2451
Illegal XbaI site found at 3788
Illegal XbaI site found at 3847
Illegal NgoMIV site found at 1349
Illegal AgeI site found at 1026
Illegal AgeI site found at 4704
Illegal AgeI site found at 5028 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |