Composite
Part:BBa_K5303109
Designed by: Dongxun Li Group: iGEM24_NNU-CHINA (2024-09-27)
pTarget-adE
The adE gene sequence was inserted behind the SRBS on the pTarget plasmid backbone. After the pTarget-adE plasmid is introduced into E.coli, the expression level of adE target genes can be upregulated.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2023
Illegal XbaI site found at 2029
Illegal SpeI site found at 1955
Illegal PstI site found at 2041 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2023
Illegal NheI site found at 1932
Illegal SpeI site found at 1955
Illegal PstI site found at 2041 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2023
Illegal BglII site found at 2053
Illegal BamHI site found at 1920
Illegal XhoI site found at 2384 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2023
Illegal XbaI site found at 2029
Illegal SpeI site found at 1955
Illegal PstI site found at 2041 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2023
Illegal XbaI site found at 2029
Illegal SpeI site found at 1955
Illegal PstI site found at 2041
Illegal NgoMIV site found at 771 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1856
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Categories
Parameters
None |