Composite

Part:BBa_K5303109

Designed by: Dongxun Li   Group: iGEM24_NNU-CHINA   (2024-09-27)


pTarget-adE

The adE gene sequence was inserted behind the SRBS on the pTarget plasmid backbone. After the pTarget-adE plasmid is introduced into E.coli, the expression level of adE target genes can be upregulated.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2023
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2041
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2023
    Illegal NheI site found at 1932
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2041
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2023
    Illegal BglII site found at 2053
    Illegal BamHI site found at 1920
    Illegal XhoI site found at 2384
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2023
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2041
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2023
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2041
    Illegal NgoMIV site found at 771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1856


[edit]
Categories
Parameters
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