Composite

Part:BBa_K5303095

Designed by: Dongxun Li   Group: iGEM24_NNU-CHINA   (2024-09-26)


pET24a-RChemA-I292A

PET24a-RChemA-I292A was constructed using the Gibson Assembly method on the pET24a vector. The DNA fragment of RChemA-I292A was subcloned into the RBS site between the T7 promoter and T7 terminator. The antibiotic marker of pET24a is the kanamycin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3112
    Illegal XbaI site found at 2514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3112
    Illegal XbaI site found at 2514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3112
    Illegal XbaI site found at 2514
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None