Composite
Part:BBa_K5303095
Designed by: Dongxun Li Group: iGEM24_NNU-CHINA (2024-09-26)
pET24a-RChemA-I292A
PET24a-RChemA-I292A was constructed using the Gibson Assembly method on the pET24a vector. The DNA fragment of RChemA-I292A was subcloned into the RBS site between the T7 promoter and T7 terminator. The antibiotic marker of pET24a is the kanamycin resistance gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3112
Illegal XbaI site found at 2514 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3112
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3112
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3112
Illegal XbaI site found at 2514 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3112
Illegal XbaI site found at 2514
Illegal NgoMIV site found at 220 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |