Composite

Part:BBa_K5303082

Designed by: Dongxun Li   Group: iGEM24_NNU-CHINA   (2024-09-26)


pET24a

The pET24a vector is suitable for various E. coli expression hosts, such as BL21 (DE3), BLR (DE3, Rosetta (DE3), and OrigamiB (DE3, etc.). We will use it as an expression vector for the hemA gene. This vector has RBS and replicons, as well as kanamycin resistance for screening and transforming bacteria. The pET24a vector can induce protein expression using the T7 promoter or LacI operon system via isopropyl - β - D-thiogalactoside (IPTG) or lactose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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