Composite
Part:BBa_K5303082
Designed by: Dongxun Li Group: iGEM24_NNU-CHINA (2024-09-26)
pET24a
The pET24a vector is suitable for various E. coli expression hosts, such as BL21 (DE3), BLR (DE3, Rosetta (DE3), and OrigamiB (DE3, etc.). We will use it as an expression vector for the hemA gene. This vector has RBS and replicons, as well as kanamycin resistance for screening and transforming bacteria. The pET24a vector can induce protein expression using the T7 promoter or LacI operon system via isopropyl - β - D-thiogalactoside (IPTG) or lactose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 220
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |