Coding

Part:BBa_K5303001

Designed by: Mengying Zhao   Group: iGEM24_NNU-CHINA   (2024-09-22)


RchemA

AFhemA is a gene from Erythrobacter podoconiosis that synthesizes ALAS.We discovered this highly active ALAS synthesis gene through literature research, and introduced its constitutive plasmid into E. coli for expression. We compared the expression effect with other sources of ALAS genes to lay the foundation for subsequent experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 616
    Illegal XbaI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 616
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 616
    Illegal XbaI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 616
    Illegal XbaI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None