Coding
Part:BBa_K5303001
Designed by: Mengying Zhao Group: iGEM24_NNU-CHINA (2024-09-22)
RchemA
AFhemA is a gene from Erythrobacter podoconiosis that synthesizes ALAS.We discovered this highly active ALAS synthesis gene through literature research, and introduced its constitutive plasmid into E. coli for expression. We compared the expression effect with other sources of ALAS genes to lay the foundation for subsequent experiments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 616
Illegal XbaI site found at 18 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 616
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 616
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 616
Illegal XbaI site found at 18 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 616
Illegal XbaI site found at 18 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |