Composite

Part:BBa_K5301027

Designed by: Ruiyao Xu   Group: iGEM24_BNU-China   (2024-09-25)


sGFP11 tether expression pathway (T7)

This pathway expresses sGFP11 tether (BBa_K5301018) using T7 expression system，containing T7 promoter, mSA, sGFP11, and T7 terminator.

Usage and Biology

We used this pathway to express sGFP11 tether（mSA - sGFP11） by using T7 expression system in E.coli, and the expression of sGFP11 tether can be induced by IPTG. For more information on sGFP11 tether[1-10], go to BBa_K5301018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None