Composite

Part:BBa_K5301020:Design

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-24)


SnCSdT expression pathway (T7)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1081
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1223
    Illegal PstI site found at 1081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BglII site found at 635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1081
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1081
    Illegal AgeI site found at 160
    Illegal AgeI site found at 920
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac operator helps it to express in T7 system.

Source

It's genetically engineered to produce large nanodiscs more conveniently .

References