Composite
Part:BBa_K5301020
Designed by: Jiaxin Guan Group: iGEM24_BNU-China (2024-09-24)
SnCSdT expression pathway (T7)
This pathway expresses SnCSdT using T7 expression system.
Usage and Biology
We used this pathway to express SnCSdT by using T7 expression system in E.coli, and the expression of SnCSdT can be induced by IPTG. For more information on SnCSdT, go to BBa_K5301006.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1081
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1223
Illegal PstI site found at 1081 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 263
Illegal BglII site found at 635 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1081
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1081
Illegal AgeI site found at 160
Illegal AgeI site found at 920 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |