Coding

Part:BBa_K5301017:Design

Designed by: Ruiyao Xu   Group: iGEM24_BNU-China   (2024-09-23)


sGFP1-10 tether is composed of mSA, a 3C linker, a 6His tag, and sGFP 1-10.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct membrane protein dimers through the interaction between biotin and streptavidin, as well as the self-assembly mechanism of sGFP.


Source

The mSA sequence comes from BBa_K4623001 from the 2023 BNU-China team, and the sGFP1-10 sequence comes from GFP.

References