Plasmid

Part:BBa_K530039

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)

pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
    Illegal NgoMIV site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 3356
    Illegal SapI site found at 2273
    Illegal SapI.rc site found at 932


Plasmid Map

PRSBB426.png

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Parameters
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