Plasmid

Part:BBa_K530036

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)

pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1183
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
    Illegal BglII site found at 959
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5258
    Illegal PstI site found at 1183
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3428
    Illegal SapI site found at 2345


Plasmid Map

PRS423.png

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Categories
Parameters
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