Regulatory

Part:BBa_K530018:Design

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-09-03)


Gal 1/10 Yeast Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 285
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.



Source

Genome sequence.

References